For polyadenylation of in vitro transcribed RNA to improve translation initiation efficiency. Contains sufficient reagents for 25 reactions.

•Add a poly(A) tail of at least 150 nucleotides in length to the 3' end of RNA
• Improve in vivo translation efficiency
• Can Adjust reactions to control tail length
• Optimized for use with RNA transcripts synthesized using the mMESSAGE mMACHINE™ Kit

PolyA Tailing Kit for use with E. coliPolyadenylate polymerase I (E-PAP) polyadenylates the 3' end of in vitro transcribed RNA. Polyadenylation plays an important role in stabilizing RNA in eukaryotes and improves translation initiation efficiency. In the PolyA Tailing Kit, theE-PAP reaction has been optimized to efficiently tail the mRNA with at least 150 adenines. The additional adenine residues confer stability to the mRNA and increase the translation efficiency of in vitro synthesized cap RNA in microinjection and transfection experiments (see figure).

Companion Products:
The PolyA Tailing Kit is optimized for use with Ambion's mMESSAGE mMACHINE™ High-Yield Cap RNA Transcription Kit. mMESSAGE mMACHINE™ Kits are available with T7 (SKU#AM1334), T3 (SKU#AM1338) or SP6 (SKU#AM1330) polymerase.