pcDNA™3.1 Targeted TOPO™ Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D/V5- His-TOPO™, enables five-minute directional cloning and subsequent high-level expression.

Directional cloning technology facilitates expression experiments because:

• Uses proofreading enzymes to reduce errors in cloned genes
• More than 90% of Clones can be cloned in the correct direction of expression, reducing time spent screening colonies for cloning direction

In addition, pcDNA3.1D/V5-His-TOPO™ provides strong expression levels from the CMV promoter and C-terminal V5-His fusion tag options for easy detection of recombinant proteins using anti-V5 antibodies and rapid protein purification on nickel chelating resin.