Flp-In™ Cell Line is designed for rapid generation of stable cell lines to express proteins of interest from Flp-In™ expression vectors . These cells contain a stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of Flp-In™ expression vectors ensures high-level expression of your gene of interest. There are six Flp-In™ cell lines available for the generation of isogenic stable cell lines and one Flp-In™ T-REx™ cell line for the generation of tetracycline-regulated stable cell lines. Flp-In™-CV-1, Flp-In™-293, Flp-In™-BHK, Flp-In™-Jurkat and Flp-In™-3T3 cell lines were generated using pFRT/lac< /i>Zeo transfects parental cell lines and selects for stable Zeocin™-resistant clones. The Flp-In™-CHO cell line was generated by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 cell line contains stably integrated pFRT/lacZeo and pcDNA™6/TR (from the T-REx™ System). Co-transfection of Flp-In™ cell lines using the Flp-In™ expression vector and the Flp recombinase vector pOG44 enables targeted integration of the expression vector into the same site in each cell, ensuring uniform levels of gene expression.

Choose your Flp-In™ Vector/cell line combination
Flp-In™-CV-1, Flp-In™-293 , Flp-In™-CHO and Flp-In™ Jurkat cell lines (Figure 1) and Flp-In™ vectors expressing CMV promoter genes (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™ and pSecTag /FRT/V5-His-TOPO™). Flp-In™-BHK and Flp-In™-3T3 cells tend to downregulate the CMV promoter. Therefore, it is recommended to use Flp-In™ vectors containing the EF-1α promoter (pEF5/FRT/V5-DEST™ and pEF5/FRT/V5-D-TOPO™) with these cell lines.