Thermo Scientific phiX174 is an earlier DNA virus discovered to have a single-stranded, circular genome. The length of phiX174 DNA is 5386 nt. The DNA strand packaged into the virion is called the “+” strand. Once inside the cell, Thermo Scientific phiX174 DNA is used as a template for negative-strand synthesis, producing double-stranded DNA. Conversion of the +DNA strand to double-stranded DNA does not require any phage genes to function.

The double-stranded DNA can then be transcribed to synthesize the phage-encoded protein. Synthesis of single-stranded (+) DNA requires the phage-encoded gene A protein. DNA synthesis is initiated at the ori (+) position and proceeds in the direction indicated. Late in infection, the single strand is loop-encapsulated into new virions. The cycle is terminated by cell lysis mediated by a protein encoded by phage gene E.

Product Advantages

•Purified by chromatography using proprietary patented technology
•Over 90% supercoiled form
•From infection phiX174 am3 Isolation from Escherichia coli HF4704

Application

•Preparation of DNA molecular weight standards

GenBank/EMBL accession number
V01128, J02482, M10348, M10379, M10714, M10749, M10750, M10866, M10867, M24859

Notes on phiX174 plasmid usage:

For DNA sequences, For sequence analysis and map creation, see REviewer free online tool