pcDNA5/TO is a 5.7 kb expression vector designed for use with the T-REx system (Cat. Nos. K1020-01 and K1020 -02) and Expi293 induction platform (Cat. Nos. A39251 and A39252). It utilizes the complete CMV promoter and adds control elements from the bacterial tetracycline resistance operon, which can effectively inhibit and induce transcription from a known strong mammalian promoter sequence.

The pcDNA5/TO vector allows tetracycline-regulated expression of genes of interest in mammalian host cells expressing the Tet repressor (TetR) from the pcDNA6/TR vector (Cat. No. V1025-20).