Features of Fish Serum Blocking Buffer include:
•Non-mammalian—Fish proteins are unlikely to interact specifically with antibodies and other mammalian proteins used in conventional methods
• Convenient—Filtered and stable in PBS, compatible with most assay systems
• Easy to use—Can be used as is or diluted 10x as needed
>• Flexible—Can be used in many different applications, including as an antibody diluent

Fish serum offers several advantages over typical blocking buffers. Because salmon is more distantly related to mammals, the source of most of the antibodies and samples used in the experiments, its serum proteins are unlikely to have specific binding interactions with the proteins used in the experiments. Fish serum blocking buffer effectively stabilizes solutions and samples used to generate antibody binding interactions while minimizing the potential for cross-reactivity with mammalian sample components. Try using this when using more standard sera in IHC will produce background staining. Additionally, this blocking agent is particularly effective in a variety of cell imaging protocols based on visible and near-infrared fluorescence detection. It has been used with near-infrared fluorescent probes to reduce background and increase signal-to-noise ratio.