Thermo Scientific DRAQ5™ fluorescent probe is a far-infrared DNA stain that can be used on live or fixed cells and can Easily perform multi-pass detection by combining other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins.

DRAQ5™ Fluorescent Probe is a far-infrared DNA stain for fluorescent cell imaging applications using live or fixed cells. Due to its far-infrared excitation and emission spectra, DRAQ5 stain can be combined with a variety of other fluorophores for multi-channel detection. In cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins, it is common to choose to counterstain nuclei and measure DNA content. DRAQ5 fluorescent probes are compatible with many existing protocols on a variety of instrument platforms.

DRAQ5 fluorescent probe features:

•DRAQ5 dye — a red-infrared fluorescent DNA special stain (quantitative nuclei and DNA)
• < b>Convenient — Available in an easy-to-use DRAQ5 dye solution (5 mM)
• Cell Imaging — Cell membrane-permeable lipophilic dye for efficient imaging of both live and fixed cells Staining and quantification of DNA content
• Counterstain — Ideal for use with other fluorophore reporters for fluorescence microscopy or high content screening (HCS) analysis

Applications:
• Flow Cytometry
• Flow and Laser Scanning Cell Analysis
• Live Cell Imaging
• Cellular Assays

DRAQ5 fluorescent dye properties
• Structure: Patented structure
• Alias: Anthraquinone compound
• Chemical name: 1,5-bis{[2-(dimethylamino)ethyl ]Amino}-4,8-dihydroxyanthracene-9,10-dione
• Molecular formula: None
• Molecular weight: 412.54
• Excitation wavelength: 647 nm Excellent spectral line (Exλmax = 646 nm)
• Emission wavelength: > 665 nm to infrared> 800 nm (Emλmax = 681 nm/697 nm, incorporated with dsDNA)
• Extinction coefficient: None
• CAS number: Temporary None
• Purity: > 99 %
• Solubility: soluble in water and common buffers at working concentrations
• Reactive groups: None; can bind DNA AT minor groove, simulate histone 2b distribution, Forming a 16 nm red shift

Fluorescent cellular assays such as flow cytometry, intracellular ELISA, fluorescence microscopy, and high-content imaging require the use of fluorescent markers to identify individual cells. When using multiple fluorescent probes to detect different cellular targets or activities, each probe must have a different fluorescence spectrum than the others. Blue fluorescent DNA-binding probes Hoechst and DAPI is the most commonly used fluorescent probe; unfortunately, when UV illumination is not available or when other blue-emitting fluorescent probes are used, these Probe is not available. Therefore, nuclear probes that emit non-blue light facilitate cell identification and enumeration as well as determination of nuclear morphology and DNA content

DRAQ5 fluorescent probes emit during far-infrared wavelengths, are lipophilic, and can pass through living cells, Fix cell and nuclear membranes in cells and tissues for rapid DNA staining. The stain is supplied as a ready-to-use aqueous solution that requires no RNase treatment, cell lysis or washing steps and is compatible with automated applications. Because DNA stains in a stoichiometric format, DRAQ5 fluorescent probes can be used to analyze DNA content in cell proliferation studies. Since no washing is required, the DRAQ5 fluorescent probe is often added as the last stain in the labeling procedure. In addition, it can also be added to the detection medium in live cell analysis experiments.