Thermo Scientific NHS-Phosphine is an amine-reactive compound that can be used to derive primary amines or amine-coated polymers of proteins surface to attach to the azide labeling molecule.

Characteristics of NHS-Phosphine:

•Solubility—The reagent is readily soluble in water-soluble solvents and is used in aqueous reactions with cell lysates and other biological samples Subsequent dilutions in mixtures
•Compatibility—Chemical reactions are performed efficiently under simple buffer conditions; no auxiliary reagents such as copper or reducing agents are required
• Specificity —NHS esters are designed to be covalently bound to primary amines (side chains of lysine or N-termini of polypeptides)
• Chemical Selectivity—Azide and phosphine groups Does not react with components of biological samples or interfere with such components (bioorthogonality), but efficiently couples to each other

This NHS-ester compound reacts with primary amines (e.g., lysine residues side chains or aminosilane-coated surfaces) react to form covalent bonds. The phosphine group (PPh2) biology-resource-library/pierce-protein-methods/staudinger-ligation-reaction-chemistry.html">Staudinger Chemistry's mechanism reacts with azide-tagged molecules to enable efficient and specific coupling of biological samples derived molecules. This NHS-phosphine reagent combined with different azide labeling compounds facilitates various labeling and cross-linking experimental strategies for studying protein interactions and cellular pathways.

Related products
NHS-phosphine< br>NHS-azide