ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit contains ViraPower™ HiPerform™ Promoterless Lentivirus for MultiSite Gateway™ The expression vector pLenti6.4⁄R4R2⁄V5-DEST™ is used for simple recombination-based cloning and lentivirus-based high-level expression of genes of interest that can be selected from any promoter from dividing and non-dividing mammalian cells. The pLenti6.4⁄R4R2⁄V5-DEST™ vector is equipped with two key genetic elements that make it a HiPerform™ vector: the woodchuck post-transcriptional regulatory element (WPRE) from the HIV-1 integrase gene and the central polypurine pipeline ( cPPT) sequences, increasing protein expression by at least 4-fold (compared to vectors lacking these elements).

Advantages
•Generate replication-incompetent lentivirus for transduction of dividing and non-dividing mammalian cells
•Use MultiSite Gateway™ technology to simply Recombine and clone multiple DNA fragments into the vector in a specific order and direction
•Express the target gene under the control of a self-selected promoter
•Stable, long-term expression
•Compared with traditional lentiviral expression systems , protein expression increased by at least 4 times

Main Features
• The WPRE of woodchuck hepatitis virus increases the transgene expression and cPPT of the HIV-1 integrase gene, increasing the The number of copies of the lentivirus integrated into the host genome increases viral titer. Combining WPRE and cPPT resulted in at least a fourfold increase in protein expression in most cell types compared to other vectors without these elements.
•Promoterless vector is used to express target genes under the control of a self-selected promoter
•Blasticidin selection marker is used for stable screening under the control of the PGK promoter to obtain long-term, sustained expression< br>
Kit includes
• pLenti6.4⁄R4R2⁄V5-DEST™ Kit
• pENTR™ 5' TOPO™ TA Cloning Kit (Cat. No. K59120)< br>•One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)
•pENTR™ Gus Positive Control Plasmid

Research use only. Not for therapeutic or diagnostic use.