Thermo Scientific Pierce Co-Immunoprecipitation Kits provide covalent antibody immobilization and the tools needed to perform properly controlled co-IP experiments All components, no antibody interference in the finished product.

Co-IP is a common method for studying protein:protein interactions, which uses antibodies to immunoprecipitate the antigen (bait protein) and co-immunoprecipitate any interacting protein (prey protein). Traditional co-IP methods using protein A or G can result in co-elution of antibody heavy and light chains, which may comigrate with associated bands, masking important results. The Pierce Co-IP kit solves this problem by covalently coupling the antibody to an amine-reactive resin. The kit contains optimized buffers for protein binding and recovery, reagents needed to perform control experiments, and high-efficiency spin columns and collection tubes to shorten protocols and minimize handling and mixing.

Co-immunoprecipitation kit features:

•Works with any purified antibody— Use any purified antibody for co-IP experiments, regardless of species or Ig class ( Chicken IgY, human IgE, mouse IgG1, IgM, etc.); affinity binding independent of protein A and protein G
• No antibody interference—Covalent binding method and non-reducing elution The system produces a pure co-IP product that is not contaminated by IP antibodies
• Reusable Antibody Beads— Covalent antibody immobilization allows reuse of prepared IP resin and Possible savings on expensive antibodies
• Complete kit—All reagents for antibody immobilization and many IP experiments, including binding and wash buffers, elution buffers, and convenient microspin columns, making Resin handling is simpler and more efficient.
•Control beads included—Underivatized agarose beads are provided for use as a negative control for non-specific binding
• Universal—co-IP The method is compatible with any physiological (non-denatured) protein sample buffer that is compatible with specific antibody-antigen and protein-interacting complexes.

Co-immunoprecipitation (Co-IP) is a commonly used in vitro method for discovering protein interactions. The Pierce Co-Immunoprecipitation Kit is configured to be an essential tool for efficient Co-IP experiments, producing pure IP and co-IP products without troublesome IP antibodies. This improvement over traditional co-IP technology is achieved by replacing Protein A/G Sepharose beads with AminoLink Plus resin, which directly and permanently couples pure IP antibodies. Mild (non-reducing, non-denaturing) elution buffer allows dissociation of IP targets while retaining antibodies immobilized on agarose beads. The result is a pure product that can be analyzed by Western blotting (or even sequencing) without antibody interference.

In addition to eliminating IP antibody contamination, the antibody conjugation method that makes up the Pierce Co-IP kit also avoids non-specific binding interactions and leaching contamination from traditional Protein A and Protein G methods. Although the conjugation method requires that the starting antibody be contained in an amine-free buffer free of BSA, gelatin, and other storage protein stabilizers, the fact that it is not dependent on protein A or protein G means that any antibody species or class can be used on IP (e.g., IgM and chicken IgY). Virtually any purified protein can be immobilized for affinity purification of binding partners.