Ham's F-12 Nutrient Blend (F-12) Blood-free list of cells designed for use with Chinese Hamster Ovary (CHO) cells decking. Since then, F-12 has been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
Full formulation is available.

F-12 contains a wider range of ingredients than other basal media, including zinc, putrescine, hypoxanthine and thymidine. Serum-free growth of CHO cells in F-12 has led to various modified formulations. Contains GlutaMAX™ Supplemented Gibco™ F-12 Medium comes in an easy-to-use packaged format that significantly reduces the accumulation of toxic ammonia and improves cell viability and growth.

Use Ham's F-12 Nutritional Blend
Gibco™ F-12 does not contain protein or growth factors. Therefore, F-12 requires additives, usually 10% -Culture/fbs.html">Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

cGMP Production and Quality Systems
To ensure the stability of the supply chain, Gibco™ F-12 is produced simultaneously in two independent factories in Grand Island, New York and Scotland, United Kingdom . Both factories comply with cGMP production requirements, are ISO 13485 certified, and are FDA-registered medical device manufacturers.